Application of Peristaltic Pumps in Chromatography Experiments

2023-04-21 14:58:01

Chromatography is a class of mixture separation techniques widely used in analytical chemistry, organic chemistry, biochemistry, and other fields. Chromatography separates and analyzes multi-component mixtures by exploiting the differences in the physical and chemical properties of the components. According to the mechanism, chromatography can be classified into adsorption chromatography, partition chromatography, ion exchange chromatography, gel filtration chromatography, and others.

Specific applications of chromatography include:

- Desalting, such as removing low-molecular-weight impurities from solutions of macromolecules (such as proteins, nucleic acids, polysaccharides, etc.) using gel filtration

- Separation and purification, applied to the purification of enzymes, proteins, polysaccharides, hormones, alkaloids, and other substances

- Concentration of dilute solutions

- Determination of the molecular weight of macromolecules

- Studies on various chromatography methods and the performance of stationary phases

In natural conditions, due to the limited permeation rate of liquids in chromatography columns, appropriate external pressure is needed to enhance the permeation capability of the liquids. At the same time, it is also necessary to ensure that the liquids do not cross-contaminate. Therefore, peristaltic pumps are an ideal choice for fluid delivery in chromatography processes. They are commonly used to control the flow rate of the mobile phase, the flow rate of the eluent, pump buffer solutions, and withdraw samples for analysis. Recommended product: BT101S.

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