OEM peristaltic pump in laboratory small fermenter fermentation experiment

It is generally believed that less than 500L is a laboratory-scale fermenter, and more than 500L is a production-scale fermenter. Small fermentation tanks in the laboratory are the prototype and foundation of industrial fermentation tank fermentation, providing data information for production-scale fermentation tank operation, including: seed verification, media formulation screening, process parameter optimization, etc. Therefore, doing a good job in the fermentation of small laboratory fermenters is the only way to put them into industrial production in the future!

Laboratory-scale fermenters are generally equipped with four peristaltic pumps for each reaction vessel, and the OEM products of fixed speed peristaltic pumps are generally selected, and the control system controls the amount of addition by controlling the start and stop time of the peristaltic pump, and the typical product is OEM03-TH15.

Two of the peristaltic pumps add acid and alkali: the control system controls the PH value of the reaction environment at the set target value through PH value monitoring and closed-loop control of adding acid or alkali.

All the way peristaltic pump adds defoamer: the control system controls the amount of foam in the reaction environment at the set target value through foam monitoring and closed-loop control of adding defoamer.

The other peristaltic pump adds culture medium: the control system controls the nutrient level of the reaction environment at the set target value through nutrient level monitoring and closed-loop control of adding nutrient reagents.

In the fermentation tank fermentation test, the fermentation medium is used for the growth, reproduction and synthesis of the bacteria. It should not only make the seeds grow rapidly after inoculation and reach a certain concentration of mycelium, but also enable the grown bacteria to quickly synthesize the required products. Therefore, it is required that the composition of the fermentation medium should be rich and complete, and the carbon and nitrogen sources should pay attention to the combination of fast and slow effects, and use less fast and slow nutrition. also consider the appropriate carbon-nitrogen ratio and add buffer to stabilize the pH value; and there must also be growth factors required for bacterial growth and elements, precursors and accelerators required for product synthesis. In addition to the elements and compounds necessary for the growth of the bacteria, there must also be specific elements, precursors and accelerators required for the product. However, if the concentration of the total carbon, nitrogen, phosphorus, etc. required by the growth and biosynthesis products is too high, or the optimal conditions required for the two stages of growth and synthesis are different, the medium can be considered to be met by batch feeding.

Reagents such as defoamers are also essential, because when conducting fermentation experiments, microorganisms will definitely produce metabolites, which will cause changes in the pH of the bacterial solution, and with the progress of fermentation, a large number of bubbles will definitely be produced, so that the dissolved oxygen of the bacterial solution will decrease, resulting in the inability of bacteria to grow and reproduce normally. At this time, acid and alkali are needed to adjust its pH, and defoamers are used to eliminate a large number of bubbles to achieve the most suitable growth environment.